《植物生理学报》 2013, 49(3): 259-263

三种抗氧化剂对曼地亚红豆杉愈伤组织褐化及相关物质含量的影响

郑超, 徐晟, 夏冰, 郑玉红, 贺佳, 彭峰, 汪仁*

江苏省中国科学院植物研究所, 南京210014

通信作者：汪仁；E-mail: jswangren@yahoo.com.cn；Tel: 025-84347111

摘　要：

采用不同浓度聚乙烯吡咯烷酮(PVP)、维生素C (VC)、柠檬酸3种抗氧化剂处理褐化的曼地亚红豆杉愈伤组织, 检测结果表明, 在柠檬酸5 mg·L-1、VC 10 mg·L-1或PVP 5 mg·L-1处理下总酚含量为最低, VC、柠檬酸处理后总酚含量下降显著,分别比对照减少77.8%、87.4%, 而PVP处理后下降不显著; 与愈伤组织褐化相关的多酚氧化酶(PPO)和过氧化物酶(POD),在PVP浓度为2.5和10.0 mg·L-1时活性分别达到最低, 与对照相比下降72.6%和97.8%。相关性分析结果表明, 愈伤组织褐化程度与总酚含量呈显著正相关, 而总酚含量与PPO、POD活性则呈负相关。可见, 抗氧化剂VC和柠檬酸处理红豆杉愈伤组织, 能有效抑制总酚的积累, 降低其褐化程度

关键词：曼地亚红豆杉; 愈伤组织褐化; 总酚含量; POD; PPO

收稿：2012-12-26   修定：2013-02-06

资助：国家高技术研究发展计划(“863”计划)子课题(2011AA-10A206)、江苏省农业自主创新资金项目[CX(12)2041]、江苏省苏北发展计划(BN2012078)。

Effects of Three Antioxidants on Callus Browning and Its Related Substance Contents in Taxus media Rehder

ZHENG Chao, XU Sheng, XIA Bing, ZHENG Yu-Hong, HE Jia, PENG Feng, WANG Ren*

Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014, China

Corresponding author: WANG Ren; E-mail: jswangren@yahoo.com.cn; Tel: 025-84347111

Abstract:

Different concentrations of polyvinylpyrrolidone (PVP), vitamin C (VC) and citric acid were performed on callus browning of Taxus media. The result showed that the total phenolic content was the lowest at 5 mg·L^-1 of citric acid, 10 mg·L^-1 of VC or 5 mg·L^-1 of PVP. Compared with the control, the total phenolic content was significantly decreased by 77.8% and 87.4% after treatment with VC and citric acid. Additionally, activities of callus browning-related enzymes PPO and POD were reduced by 72.6% and 97.8% at their lowest levels under treatments with 2.5 and 10.0 mg·L^-1 PVP respectively. The correlation analysis among the browning degrees of callus, total phenolic content, PPO and POD activities was also carried out. A significant positive correlation between total phenolic content and callus browning was found. But total phenolic content was negatively correlated to PPO and POD activities. It indicates that VC and citric acid are more effective to inhibit total phenolic accumulation in T. media callus, and thus reducing callus browning.

Key words: Taxus media; callus browning; total phenolic content; POD; PPO